急性高血糖对大鼠脑缺血再灌注损伤的影响

被引：1

作者：

毛伦林 ^{[1
]}

管阳太 ^{[1
]}

毛晓薇 ^{[1
]}

张秀天 ^{[1
]}

顾桢茂 ^{[2
]}

机构：

[1] 第二军医大学附属长海医院神经内科

[2] 上海市脑血管病防治研究所

来源：

中国动脉硬化杂志 | 2012年 / 20卷 / 08期

关键词：

急性高血糖; 脑缺血再灌注损伤; 肌酸激酶脑型同工酶; 大鼠;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

1002 ;

摘要：

目的探讨急性高血糖对大鼠脑缺血再灌注损伤的影响,并分析高血糖浓度与脑缺血再灌注损伤程度及预后关系。方法制备SD大鼠大脑中动脉缺血再灌注模型,于术前30 min腹腔注射25%葡萄糖或生理盐水(10 mL/kg体重),缺血90 min,再灌注24 h后评估大鼠神经缺失症状,检测血清肌酸激酶脑型同工酶水平、脑梗死体积、脑组织含水量,以及神经细胞凋亡和脑组织病理形态学改变。结果与模型组相比,高血糖组大鼠急性期存活率降低(P<0.05),脑组织病理损害明显加重,脑梗死体积、细胞凋亡数、脑组织含水量、颅底出血风险、神经缺失症状及血清肌酸激酶脑型同工酶水平均显著升高(P<0.05或P<0.01)。大鼠血清肌酸激酶脑型同工酶水平与缺血再灌注前血糖浓度有直线相关关系(r=0.68)。结论通过扩大脑梗死体积,促进细胞凋亡,增加脑组织含水量和血清肌酸激酶脑型同工酶水平,急性高血糖加重大鼠脑缺血再灌注损伤。高血糖浓度与脑缺血再灌注损伤程度有直线相关关系,对预后不利。

引用

页码：698 / 704

页数：7

共 29 条

[1] REVERSIBLE MIDDLE CEREBRAL-ARTERY OCCLUSION WITHOUT CRANIECTOMY IN RATS [J].

LONGA, EZ ;

WEINSTEIN, PR ;

CARLSON, S ;

CUMMINS, R .

STROKE, 1989, 20 (01) :84-91

[2]

Neurology[C]. The 5th International Congress of Pathophysiology,2006

[3] Hyperbaric oxygen-induced attenuation of hemorrhagic transformation after experimental focal transient cerebral ischemia [J].

Qin, Zhiyong ;

Karabiyikoglu, Murat ;

Hua, Ya ;

Silbergleit, Robert ;

He, Yangdong ;

Keep, Richard F. ;

Xi, Guohua .

STROKE, 2007, 38 (04) :1362-1367

[4] Effects of admission hyperglycemia on mortality and costs in acute ischemic stroke [J].

Williams, LS ;

Rotich, J ;

Qi, R ;

Fineberg, N ;

Espay, A ;

Bruno, A ;

Fineberg, SE ;

Tierney, WR .

NEUROLOGY, 2002, 59 (01) :67-71

[5]

Enzymatic changes in serum and cerebrospinal fluid in neurological injury. Bakay RAE,Ward AA. Journal of Neurosurgery . 1983

[6] EFFECT OF PLASMA-GLUCOSE ON INFARCT SIZE IN FOCAL CEREBRAL ISCHEMIA-REPERFUSION [J].

YIP, PK ;

HE, YY ;

HSU, CY ;

GARG, N ;

MARANGOS, P ;

HOGAN, EL .

NEUROLOGY, 1991, 41 (06) :899-905

[7]

Influence of Hyperglycemia on Oxidative Stress and Matrix Metalloproteinase-9 Activation After Focal Cerebral Ischemia/Reperfusion in Rats: Relation to Blood-Brain Barrier Dysfunction[J] . Hiroshi Kamada,Fengshan Yu,Chikako Nito,Pak H. Chan. &nbspStroke . 2007 (3)

[8]

Hyperbaric oxy-gen-induced attenuation of hemorrhagic transformation afterexperimental focal transient cerebral. Qin Z,Karabiyikoglu M,Hua Y,et al. Stroke . 2007

[9]

Reversible middle cerebral artery occlusion without crainectomy in rats. Longa EZ,Weinstein PR,Carlsons S,et al. Stroke . 1989

[10]

The relationship between glucose and incident cardiovascular events. A metaregression analysis of published data from 20 studies of 95,783 individuals followed for 12.4 years. Coutinho M,Gerstein HC,Wang Y,et al. Diabetes Care . 1999

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