缺氧诱导离体心肌细胞凋亡的动物实验研究

被引：4

作者：

徐德民

赵强

陈安清

赵军

郑世营

机构：

[1] 复旦大学附属中山医院心脏外科

[2] 苏州大学附属第一医院心胸外科

来源：

中国临床医学 | 2004年 / 05期

关键词：

缺氧; 鼠心肌细胞; 流式细胞术; DNA琼脂糖凝胶电泳; 心肌细胞凋亡;

D O I：

暂无

中图分类号：

R541 [心脏疾病];

学科分类号：

摘要：

目的 :建立一种新的SD乳鼠心肌细胞的缺氧模型 ,以探讨心肌细胞缺氧损伤后的细胞凋亡及其时序分布特点。方法 :SD乳鼠的培养心肌细胞随机分为 5组 :对照组 ,缺氧 12h组 (I12h)、缺氧 2 4h组 (I4h) ,缺氧 4 8h组 (I4 8h)以及缺氧 72h组(I72h)。应用流式细胞术、DNA琼脂糖凝胶电泳、透射电镜等多种手段检测心肌细胞凋亡的改变。结果 :在本实验的心肌细胞缺氧模型中 ,流式细胞仪检测发现 ,心肌细胞缺氧后的细胞凋亡百分率迅速增高。琼脂糖凝胶电泳分析 ,心肌细胞在缺氧4 8h、72h时均出现了凋亡特异性的“梯状”电泳带。结论 :本实验培养乳鼠心肌细胞缺氧模型可导致心肌细胞凋亡 ,凋亡程度和心肌细胞缺氧时间有关 ,并可导致和在体心肌缺血相似的心肌损伤。细胞凋亡在心肌缺氧心肌细胞死亡中起重要作用。

引用

页码：714 / 716

页数：3

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