贯叶连翘对PC12细胞缺血性损伤的保护作用

被引：4

作者：

戴晓鸣 ^{[1
]}

孙蓉 ^{[2
]}

武栋栋 ^{[3
]}

吴玉林 ^{[1
]}

机构：

[1] 中国药科大学生理教研室

[2] 山东省中医药研究院

[3] 中科院上海药物研究所

来源：

中国临床药理学与治疗学 | 2006年 / 01期

关键词：

贯叶连翘; PC12细胞; 缺血性损伤; 线粒体跨膜电位; 细胞凋亡;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

1008 ;

摘要：

目的:研究贯叶连翘对PC12细胞缺血性损伤的保护作用。方法:在离体培养的PC12细胞,用NaCN加缺糖造成拟缺血损伤模型,噻唑蓝(MTT)、LDH活力测定、细胞内肌酸激酶(CK)活力测定、流式细胞仪测定细胞凋亡率和线粒体跨膜电位。结果:在10-7～10-5mol.L-1范围内,贯叶连翘浓度依赖地增加MTT比色值,降低缺血性损伤所致培养介质内LDH的释放,增加细胞内CK活力。贯叶连翘还可剂量相关性地降低PC12细胞的凋亡百分率和稳定细胞线粒体跨膜电位。结论:贯叶连翘对PC12细胞缺血性损伤具有保护作用,其机制可能与增加细胞内CK活力和稳定细胞线粒体跨膜电位而抑制缺血性损伤诱导的PC12细胞的凋亡有关。

引用

页码：51 / 54

页数：4

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