核因子-κB受体活化因子配体在大鼠正畸牙压力侧骨改建中的作用

被引：5

作者：

杨健

谭颖徽

裘松波

机构：

[1] 第三军医大学新桥医院口腔科,第三军医大学新桥医院口腔科,第三军医大学新桥医院口腔科重庆,重庆,重庆

来源：

口腔医学研究 | 2004年 / 03期

关键词：

核因子-κB受体活化因子配基; 骨改建; 破骨细胞;

D O I：

10.13701/j.cnki.kqyxyj.2004.03.013

中图分类号：

R783.5 [口腔正畸学];

学科分类号：

100302 ;

摘要：

目的 :初步探讨在正畸牙移动压力侧核因子 -κB受体活化因子配基 (receptoractivatorofnuclearfactor-κBligand ,RANKL)的表达在破骨细胞诱导和骨改建中的调节作用。方法 :建立大鼠正畸牙移动模型 ,利用免疫组化的方法对压力侧RANKL的表达及其变化进行检测 ;并进一步观察了RANKL对大鼠骨髓破骨细胞形成的影响。结果 :正畸牙移动压力侧组化结果显示 ,RANKL阳性表达位于牙周膜细胞和位于骨陷窝内的破骨细胞中 ,在正畸牙移动第 3、5和 7天时呈强阳性表达。体外破骨细胞诱导实验结果显示 ,在巨噬细胞集落刺激因子 (macrophageclonestimulatingfactor,M -CSF)协同作用下 ,RANKL呈剂量依赖型诱导TRAP阳性细胞生成。结论 :大鼠正畸牙移动中 ,RANKL在压力侧的表达上调有诱导破骨细胞形成的作用 ,并导致牙槽骨吸收

引用

页码：259 / 262

页数：4

共 10 条

[1]

Methodsforthepurification,assay,characterizationandtargetbindingofacolony-stimulatingfac tor（CSF -1）. StanleyER,Guilbert. Journal of Immunological Methods . 1981

[2]

Bone[P]. BIRL JOSEPH J.中国专利:US2436283A,1948-02-17

[3]

Cataboliceffectsofcon tinuoushumanPTH （1--38）invivoisassociatedwithsus tainedstimulationofRANKLandinhibitionofosteoprotegerinandgene-associatedboneformation. MaYL,CainRL,HalladayDL ,etal. The Journal of Endocrinology . 2001

[4]

Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerinligand. Hsu H,Lacey DL,Dunstan CR, et al. Proceedings of the National Academy of Sciences of the United States of America . 1999

[5]

Measuring dental drift and orthodontic tooth movement in response to various initial forces in adult rat. King GJ , Keeling SD , Mccoy EA , et al. American Journal of Orthodontics . 1991

[6]

Osteoprotegerin:anovel secreted protein involved in the regulation of bone density. Simonet WS,Lacey DL,Dunstan CR,et al. Cell . 1997

[7]

Commitment and differentiation of osteoclastprecursor cells by the sequential expression of c-Fms and receptor activator of nuclear factor kappaB （RANK） receptors. Arai F,Miyamoto T,Ohneda O,Inada T,Sudo T,Brasel K,Miyata T,Anderson DM,Suda T. The Journal of Experimental Medicine . 1999

[8]

Protein expression and functional difference of membrane-bound and soluble receptor activator of NF-kappaB ligand:modulation of the expression by osteotropic factors and cytokines. Nakashima T,Kobayashi Y,Yamasaki S, et al. Biochemical and Biophysical Research Communications . 2000

[9]

Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Suda T, Takahashi N, Udagawa N, et al. Endocrine Reviews . 1999

[10]

Bisphosphonates promote apoptosis in murine osteoclasts in vitro and in vivo. Hughes DE, Wright KR, Uy HL, et al. Journal of Bone and Mineral Research . 1995

← 1 →