菜豆病程相关蛋白基因在重金属胁迫下的表达分析

被引：15

作者：

张玉秀

柴团耀

机构：

[1] 中国科学技术大学研究生院生物学部!北京,中国科学技术大学研究生院生物学部!北京

来源：

中国生物化学与分子生物学报 | 2000年 / 01期

关键词：

重金属; 病程相关蛋白; 菜豆;

D O I：

10.13865/j.cnki.cjbmb.2000.01.010

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

为探讨植物抗重金属的分子机理 ,差别筛选了 Hg Cl2 胁迫的菜豆 ( Phaseolus vulgaris L.)叶片 c DNA库 ,分离出一个重金属胁迫响应基因 Pv SR4克隆 .c DNA和氨基酸序列分析表明 Pv SR4编码一种细胞内病程相关蛋白 ,该蛋白具有 RNase活性 .Northern blot分析表明 Pv SR4基因在正常生长条件下的叶片中不表达 ,重金属 ( Hg、Cd、As、Zn和 Cu等 )和水杨酸能强烈地诱导其基因的表达 ,受伤也能促进该基因的转录 ,而热胁迫几乎没有调节作用 .推测 Pv SR4蛋白在诱导植物的抗逆性和抵抗重金属胁迫方面有重要作用

引用

页码：46 / 50

页数：5

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