室间隔缺损ALK3下游相关基因的初步研究

被引：7

作者：

杨德业

宋后燕

张怀勤

黄晓燕

官孝群

机构：

[1] 温州医学院附属第一医院心内科

[2] 复旦大学教育部分子医学重点实验室

来源：

生物工程学报 | 2003年 / 03期

关键词：

室间隔缺损; 基因; 骨形态形成蛋白受体;

D O I：

10.13345/j.cjb.2003.03.002

中图分类号：

R541.1 [先天性心脏血管病];

学科分类号：

摘要：

了解ALK3在心脏发育中的作用 ,探索室间隔缺损的特异相关基因及其信号传导途径。应用α MHC Cre loxP系统 ,建立了心脏ALK3基因敲除小鼠模型 ,利用PCR选择性cDNA差异显示法和基因芯片扫描 (RNAmicroar ray)的方法 ,比较对照组和试验组mRNA表达水平 ,筛选ALK3下游基因。对照组的mRNA来自α MHC Cre+ -ALK3F + 的 11.5d小鼠胚胎心脏 ,试验组的mRNA来自α MHCCre+ - ALK3F - 的 11.5d小鼠胚胎心脏。心脏特异的ALK3基因敲除后 ,血小板激活因子乙酰水解酶及转录因子Pax 8等基因的表达水平下降 ,β亚类 14 3 3蛋白及蛋白酪氨酸激酶等基因的表达水平上调。血小板激活因子乙酰水解酶及转录因子Pax 8等基因可能是ALK3重要的下游基因 ,与室间隔缺损的形成有关 ;β亚类 14 3 3蛋白及蛋白酪氨酸激酶等基因是骨形态形成蛋白信号传导途径的调控因子

引用

页码：267 / 271

页数：5

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