免疫PCR方法在血清抗Sm抗体测定中的应用

被引:1
作者
曾常茜
晏舒
王玉国
机构
[1] 北华大学医学院免疫教研室!吉林
[2] 北华大学医学院第二临床学院检验科!吉林
[3] 长春市中心医院检验科!长春
关键词
免疫PCR方法; 抗Sm抗体; SLE;
D O I
暂无
中图分类号
R446.61 [免疫学检验方法];
学科分类号
100208 ;
摘要
目的 :将免疫PCR方法应用于血清抗Sm抗体测定中。方法 :采用SaHIgG DNA探针建立了血清抗Sm抗体免疫PCR检测方法。结果 :该法测定SLE患者血清抗Sm抗体的特异性强 ,敏感性是ELISA方法的 10 7倍。批内和批间变异系数分别为 2 3%~ 4 8%和 3 7%~ 6 9% ,而且与ELISA方法呈高度正相关。结论 :免疫PCR方法用于血清抗Sm抗体测定具有临床实用价值
引用
收藏
页码:263 / 265
页数:3
相关论文
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