微生物扩增子高通量测序技术在水产品加工与贮藏中的应用

被引：21

作者：

王伟 ^{[1
,2
]}

冷凯良 ^{[1
]}

刘均忠 ^{[1
,2
]}

郝建华 ^{[1
,2
]}

孙谧 ^{[1
,2
]}

机构：

[1] 中国水产科学研究院黄海水产研究所农业部极地渔业开发重点实验室

[2] 青岛海洋科学与技术国家实验室海洋药物与生物制品功能实验室

来源：

食品与发酵工业 | 2017年 / 43卷 / 10期

关键词：

扩增子高通量测序; 16S rRNA基因; 水产品加工与贮藏; 微生物检测;

D O I：

10.13995/j.cnki.11-1802/ts.014760

中图分类号：

TS254.4 [水产食品加工与保藏];

学科分类号：

083204 [水产品加工及贮藏工程];

摘要：

简介了微生物扩增子高通量测序技术,简述该技术在水产品加工与贮藏研究中的应用现状。在发酵水产品、水产品低温加工和保鲜防腐等方向,众多研究以高通量方法测定细菌和古菌的16S rRNA基因片段,准确获得水产品中微生物的种类和数量信息。微生物扩增子高通量测序技术优于传统的可培养法,也优于许多非培养分析技术。同时还指出了该技术的存在问题及对策,并探讨了该技术的发展方向和应用前景。

引用

页码：263 / 268

页数：6

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