人参皂苷Rg1和Rh1对树突状细胞功能的影响(英文)

被引：10

作者：

王毅

郝钰

娄金丽

马辉

邱全瑛

机构：

[1] 清华大学物理系,北京中医药大学病原系,北京中医药大学病原系,清华大学物理系,北京中医药大学病原系北京,北京,北京,北京,北京

来源：

中国病理生理杂志 | 2004年 / 10期

关键词：

人参皂苷Rg1; 人参皂苷Rh1; 树突细胞; 白细胞介素-12; 肿瘤;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

1008 ;

摘要：

目的 :研究人参皂苷Rg1和Rh1对树突状细胞 (dendriticcell,DC)功能的影响。方法 :用ELISA法检测人参皂苷Rg1及Rh1对DC的IL - 12p4 0蛋白产生量的影响 ,用RT -PCR检测人参皂苷Rg1及Rh1对DCIL - 12p4 0mRNA表达水平的影响。用中性红吞噬法检测人参皂苷Rg1及Rh1对DC -LPAK对肿瘤细胞的杀伤作用。结果 :ELISA结果表明 ,Rg1和Rh1各剂量组均能显著提高DCIL - 12p4 0蛋白的产量。与对照组相比 ,Rg11mg/L及Rh110 0mg/L可明显增加DCIL - 12p4 0mRNA的转录 ,与其蛋白表达相一致。Rg1及Rh1均能促进DC -LPAK对乳头瘤细胞的杀伤能力 ;在对L92 9杀伤实验中 ,效靶比为 5∶1时 ,Rg1各剂量均能显著促进DC -LPAK的杀伤活性(P <0 0 1,P <0 0 5 ) ,而Rh1仅中剂量能提高DC -LPAK的杀伤活力 (P <0 0 5 )。结论 :Rg1和Rh1通过增强IL - 12p4 0mRNA的表达来增加其蛋白的产量。并且 ,由于IL - 12产量的增加从而增强了DC -LPAK对肿瘤细胞的杀伤能力

引用

收藏

页码：18 / 23

页数：6

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