超广谱β-内酰胺酶分子生物学基础的研究进展

被引：21

作者：

陆坚

唐英春

机构：

[1] 中山医科大学附属第三医院

[2] 中山医科大学附属第三医院广州

[3] 广州

来源：

中国抗生素杂志 | 2001年 / 06期

关键词：

超广谱β-内酰胺酶; 分子生物学; 耐药机制;

D O I：

10.13461/j.cnki.cja.002996

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

革兰氏阴性杆菌产超广谱 β-内酰胺酶 (ESBL s)是导致临床致病菌对新型广谱抗生素产生耐药性的重要机制之一。采用系统进化树的分析方法 ,对 ESBL s的氨基酸残基进行排序比较可进一步细分为 5个以上的亚群 ,同一亚群 ESBL s的功能特征具有较大的相似性。 ESBL s的产生机制可能涉及两个方面 :其一为基因转移 ,ESBL s的编码基因主要位于质粒 DNA序列中 ,由耐药基因和调控序列共同组成 ,此抗性基因盒可以通过转座子 (Tn)或整合子 (Int)及某些未知的转移机制在质粒间、质粒与染色体间乃至同种或不同种属的革兰氏阴性菌间迁移扩散 ;其二为基因变异 ,某些窄谱 β-内酰胺酶可以通过侧翼调控序列或耐药结构基因的突变 ,使其产酶效能得到大幅提高或使底物轮廓范围扩大 ,从而衍化为对多种氧亚胺基 β-内酰胺类抗生素具有催化水解效能的超广谱β-内酰胺酶

引用

页码：401 / 405+413 +413

页数：6

共 25 条

[1]

Sequences of the genes for the TEM-20, TEM-21, TEM-22, and TEM-29 extendedspectrum -lactamases. Arlet G S,Goussard P. Antimicrobial Agents and Chemotherapy . 1999

[2]

A novel class A extended-spectrum beta-lactamase ( BES-1 ) in Serratia marcescens isolated in Brazil. Bonnet R,Sampaio J L,Chanal C,et al. Antimicrobial Agents and Chemotherapy . 2000

[3]

Construction and characterization of mutants of the TEM-1 beta-lactamase containing amino acid substitutions associated with both extended-spectrum resistance and resistance to beta-lactamase inhibitors. Stapleton P D,Shannon K P,French G L,et al. Antimicrobial Agents and Chemotherapy . 1999

[4]

CTX-Mtype beta-lactamases: an emerging group of extendedspectrum enzymes. Tzouvelekis L S,Tzelepi E,Tassios P T,et al. International Journal of Antimicrobial Agents . 2000

[5]

Single amino acid replacements at positions altered in naturally occurring extended-spectrum TEM beta-lactamases. Blazquez J,Morosini M I,Negri M C,et al. Antimicrobial Agents and Chemotherapy . 1995

[6]

IBC-1, a novel integron-associated class A beta-lactamase with extended-spectrum properties produced by an Enterobacter cloacae clinical strain. Giakkoupi P,Tzouvelekis L S,Tsakris A,et al. Antimicrobial Agents and Chemotherapy . 2000

[7]

Characterization of In53, a class 1 plasmid-and composite transposon-located integron of Escherichia coli which carries an unusual array of gene cassettes. Naas T,Mikami Y,Imai T,et al. Journal of Bacteriology . 2001

[8]

First identification of an SHV-12 extended-spectrum beta-lactamase in Klebsiella pneumoniae isolated in Italy. Laksai Y,Severino M,Perilli M,et al. Journal of Antimicrobial Chemotherapy . 2000

[9]

Genetic structure and transcriptional analysis of a mobilizable,antibiotic resistance transposon from Bacteroides. Tribble G D,Parker A C,Smith C J,et al. Plasmid . 1999

[10]

Molecular basis of antibiotic resistance and beta-lactamase inhibition by mechanismbased inactivators: perspectives and future directions. Therrien C,Levesque R C. FEMS Microbiology Reviews . 2000

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