铜绿假单胞菌外膜亚蛋白质组图谱的建立

被引:3
作者
章喻军
王三英
机构
[1] 厦门大学生命科学学院
关键词
铜绿假单胞菌; 外膜蛋白; 蛋白质组学; 免疫学;
D O I
暂无
中图分类号
R378 [病原细菌];
学科分类号
100103 ; 100705 ;
摘要
铜绿假单胞菌是人类重要的病原菌,容易造成免疫缺陷患者如烧伤、艾滋病、癌症或囊性纤维化感染,对该菌外膜蛋白的研究有助于了解该菌的致病机制,为进一步疫苗研制奠定基础.采用月桂酰基氨酸钠的方法提取了铜绿假单胞菌的外膜蛋白,首次建立了外膜亚蛋白质组学表达图谱,鉴定了23个外膜蛋白,占所鉴定蛋白的82.1%.然后对其中的一个外膜蛋白基因PA3988进行了克隆及原核表达与纯化,经免疫小鼠获得抗血清后对外膜蛋白进行双向Westernblotting分析,验证了质谱的结果.这些研究结果对铜绿假单胞菌外膜蛋白的深入研究具有积极作用.
引用
收藏
页码:111 / 115
页数:5
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