低聚糖与水溶性膳食纤维对婴儿肠道菌群益生作用研究

被引：13

作者：

秦雪梅

刘静

霍贵成

机构：

[1] 东北农业大学乳品科学教育部重点实验室

来源：

食品科技 | 2011年 / 36卷 / 06期

关键词：

低聚糖; 水溶性膳食纤维; PI值; 短链脂肪酸;

D O I：

暂无

中图分类号：

TS201.4 [食品营养学];

学科分类号：

083206 [食品营养];

摘要：

目的:通过体外静置培养,分析低聚糖包括低聚果糖(FOS)、低聚半乳糖(GOS)和水溶性膳食纤维——聚葡萄糖(POL)对婴儿肠道菌群的益生作用。方法:取完全母乳喂养的新鲜婴儿粪便,加已灭菌的磷酸盐缓冲液,离心,取其上清液按10%的体积比加到培养基中,在厌氧箱中静置培养,在不同时间取样,测定婴儿肠道的菌落状态及其各种肠道菌群的数量变化,用益生指数(PI)表示;通过测定不同时间代谢产物的pH来判断低聚糖和水溶性膳食纤维的抗发酵能力,和被降解速率,最后分析代谢产物中的短链脂肪酸(SCFA)含量,综合比较它们的益生作用。结果分析,FOS、GOS、POL都有一定的益生作用,POL的抗发酵能力比较显著。

引用

页码：96 / 100

页数：5

共 9 条

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