胡萝卜人工种子在无菌条件下的贮藏及贮藏中种子活力变化

被引：6

作者：

刘凡

曹鸣庆

李修庆

机构：

[1] 北京市农林科学院蔬菜研究中心,北京市农林科学院蔬菜研究中心,北京大学生物系北京,北京,北京

来源：

实验生物学报 | 1991年 / 02期

关键词：

胡萝卜; 人工种子; 贮藏; 种子活力;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

对用海藻酸钙包埋胡萝卜体细胞胚(0.6—2mm)制作的人工种子进行贮藏研究,发现低温及干燥均能在一定程度上抑制人工种子萌发。失水率达67%的种子,2℃贮藏60天后, 在发芽培养基上发芽率为100%,7天内的成苗率达80%。未经贮藏的对照种子发芽率为100%,成苗率为76%。无论在“种皮”还是在胚胎悬浮培养液中加入脱落酸或香豆素,都能抑制胚的生长,但也促进了胚的衰老。铝箔袋内密闭贮藏期间种子不萌发,但活力下降快。随着贮藏时间的延长,种子的活力指数、脱氢酶活性、氧吸收率都呈下降趋势。

引用

页码：99 / 107

页数：9

共 11 条

[1] 胡萝卜人工种子基本制作流程的建立 [J].

李修庆 .

Journal of Integrative Plant Biology, 1989, (09) :673-677

[2]

Artificial seeds of alfalfa ( Medicago sativa L.). Induction of desiccation tolerance in somatic embryos[J] . Tissa Senaratna,Bryan D. McKersie,Stephen R. Bowley. &nbspIn Vitro Cellular & Developmental Biology . 1990 (1)

[3] STRESS-INDUCED SOMATIC EMBRYOGENESIS IN CARROT AND ITS APPLICATION TO SYNTHETIC SEED PRODUCTION [J].

KAMADA, H ;

KOBAYASHI, K ;

KIYOSUE, T ;

HARADA, H .

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY, 1989, 25 (12) :1163-1166

[4]

Artificial seeds in barley: encapsulation of microspore-derived embryos[J] . S. K. Datta,I. Potrykus. &nbspTheoretical And Applied Genetics . 1989 (6)

[5] DESICCATED QUIESCENT SOMATIC EMBRYOS OF ORCHARDGRASS FOR USE AS SYNTHETIC SEEDS [J].

GRAY, DJ ;

CONGER, BV ;

SONGSTAD, DD .

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY, 1987, 23 (01) :29-33

[6]

Durzan, 1987, Biotechnology of somatic poly-embryogenesis and plantlet regeneration in Loblolly pine. Gupta P. K. D. J. BioTech .

[7]

Harada, 1981, Changes in the endogenous level and effects of abscisic acid during somatic embryogenesis of Daucus carota L. Kamada H. H. Plant and Cell Physiology .

[8]

Skoog, 1962, A revised medium for rapid growth and bioassays with tobacco tissue cultures. Murashige T. F. Physiologia Plantarum .

[9]

Janick, 1985, Hardening treatment increased survival of synthetically-coated asexual embryos of carrot. Kitto S. L. J. Journal of the American Society for Horticultural Science .

[10]

Janick, 1985, Production of synthetic seeds by encapsulating asexual embryos of carrot. Kitto S. L. J. Journal of the American Society for Horticultural Science .

← 1 2 →