气相色谱-质谱法同时检测动物组织中多种激素类兽药的残留量

被引：42

作者：

林维宣

董伟峰

陈溪

田苗

于灵

赵景红

杨春光

机构：

[1] 辽宁出入境检验检疫局

来源：

色谱 | 2009年 / 03期

关键词：

气相色谱-质谱法; 固相萃取; 微波辅助衍生; 激素; 兽药; 多残留检测; 动物组织;

D O I：

暂无

中图分类号：

S859.84 [动物性食品中化学物残留];

学科分类号：

0906 ;

摘要：

建立了不同动物基质(肌肉、肝脏、肾脏)中己烷雌酚、己烯雌酚、己二烯雌酚、还原尿睾酮、表睾酮、雌酮、雌二醇、炔雌醇和雌三醇激素残留量的气相色谱-质谱联用(GC-MS)检测方法。以乙腈为提取溶剂,固相萃取柱净化,微波辅助衍生化,用双(三甲基硅烷基)三氟乙酰胺(BSTFA)与甲基氯硅烷(TMCS)(体积比为99:1)的硅烷化试剂在吡啶存在下进行衍生化反应。实验结果表明,9种激素的检出限为0.1～1μg/kg。3种动物基质中9种激素的平均回收率为68.8%～93.1%,实验室内相对标准偏差(RSD)为4.1%～22.3%,实验室间RSD为3.1%～17.9%。方法的技术指标满足动物组织中激素类兽药残留分析的要求。

引用

页码：294 / 298

页数：5

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