鲤和团头鲂幼鱼卵黄蛋白原的诱导、纯化及电泳比较

被引:12
作者
梁勇
徐盈
杨方星
王剑伟
机构
[1] 中国科学院水生生物研究所
[2] 淡水生态与生物技术国家重点实验室
关键词
卵黄蛋白原; 鲤科鱼类; 阴离子交换; 内分泌干扰物;
D O I
暂无
中图分类号
Q51 [蛋白质];
学科分类号
摘要
采用腹腔注射人工雌激素 1 7α 乙炔基雌二醇 (1 7α ethinyloestradiol,EE2 )的方法 ,在鲤幼鱼和团头鲂幼鱼体内诱导生成卵黄蛋白原 ;并结合利用阴离子交换介质DEAE SephraroseCL 6B和液相层析技术分离纯化了诱导后的鲤幼鱼和团头鲂幼鱼血浆中的卵黄蛋白原 ;SDS PAGE电泳结果表明所分离的两种鲤科鱼类卵黄蛋白原其亚基的分子量分别为 1 70KD和1 5 0KD。
引用
收藏
页码:317 / 321
页数:5
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