2型糖尿病患者尿液的代谢组学研究

被引：8

作者：

苏君梅 ^{[1
]}

葛卫红 ^{[1
]}

许广艳 ^{[1
]}

张宇 ^{[2
]}

机构：

[1] 浙江中医药大学药学院

[2] 浙江省中医院内科

来源：

浙江医学 | 2015年 / 37卷 / 04期

关键词：

代谢组学; 2型糖尿病; 气相色谱-质谱联用; 正交偏最小二乘法判别;

D O I：

暂无

中图分类号：

R587.1 [糖尿病];

学科分类号：

100201 [内科学];

摘要：

目的从代谢组学角度分析并寻找2型糖尿病(T2DM)患者可能的代谢标记物。方法选取30例初诊、或有糖尿病史经药物治疗控制不理想且无并发症的T2DM患者,另选取30例性别、年龄匹配的健康者为正常对照。收集清晨空腹中段尿,以气相色谱-质谱联用(GC-MS)技术对尿液样本进行代谢图谱分析,正交偏最小二乘法判别研究尿液内源性化合物在两组间的差异。结果 T2DM组和正常对照组尿液代谢谱明显分离。与正常对照组比较,T2DM组尿液2,3,4-三羟基丁酸、肌醇、D-葡萄糖、D-葡萄糖酸及尿素含量升高(P<0.05或0.01),马尿酸含量减少(P<0.01)。结论代谢组学检查提示T2DM患者尿液中代谢标志物为2,3,4-三羟基丁酸、肌醇、马尿酸、D-葡萄糖、D-葡萄糖酸及尿素,观察这些标志物含量的变化有助于T2DM的临床诊断及发病机制研究。

引用

页码：278 / 282

页数：5

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