磁性聚乙二醇载体固定化α淀粉酶的研究

被引：9

作者：

邱广亮

栗淑媛

郝翠秀

高艳春

机构：

[1] 内蒙古师范大学生物系!呼和浩特，,内蒙古师范大学生物系!呼和浩特，,内蒙古师范大学生物系!呼和浩特，,内蒙古师范大学生物系!呼和浩特，

来源：

生物化学杂志 | 1997年 / 02期

关键词：

α-Amylase; Magnetic collidal particle; Adsorbing-crosslinking; Magnetic immobilized enzyme;

D O I：

10.13865/j.cnki.cjbmb.1997.02.026

中图分类号：

Q814 [酶工程];

学科分类号：

082203 ;

摘要：

A magnetic immobilized a-amylase (MIE) has been prepared by adsorbing α-amylase onto a magnetic collidal particle and cross-linking it with glutaraldehyde. The effects of different concentrations of glutaraldehyde, pH of buffers for immobilization and the properties of magnetic immobilization α-amylase were studied. The results showed that the activity of MIE was 34000 U per gram dry gel. The protein binding capacity was 100 mg per gram dry gel. The activity recovery was 37.7%. The optimum temperature of MIE was 30 degree higher than that of natural α-amylas(50℃). The optimum pH values of MIE was 1 pH unit higher than that of natUral α-amylase. The magnetic immobilized α-amylase has excellent thermal stability and working stability.

引用

页码：240 / 242

页数：3