厚朴酚对骨关节炎的抗炎和软骨保护作用

被引：9

作者：

江晨镝 ^{[1
]}

李世政 ^{[2
]}

机构：

[1] 温州市中心医院骨科

[2] 镇远县人民医院骨科

来源：

免疫学杂志 | 2020年 / 36卷 / 05期

关键词：

骨关节炎; 厚朴酚; 炎症; 软骨;

D O I：

10.13431/j.cnki.immunol.j.20200067

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的探讨厚朴酚的体外抗炎活性和对碘乙酸单钠(MIA)诱导的骨关节炎(OA)的影响。方法脂多糖(LPS)处理的RAW264.7细胞中体外评价厚朴酚的抗炎作用,设立LPS组、厚朴酚不同浓度组(5、10、20μmol/L),以正常培养的细胞作为对照组,Griess试剂测量培养基中NO的累积;Western blot检测细胞iNOS、COX-2蛋白的表达。MIA诱导OA的SD大鼠模型,分为MIA组、厚朴酚不同浓度组(5、10、20 mg/kg),以正常大鼠作为对照组,取大鼠膝关节组织样本进行组织学分析,实时PCR分析大鼠IL-1β、TNF-α、IL-6、MMP-2、MMP-9和COX-2的mRNA表达;ELISA检测培养基上清液中PGE 2、IL-6及各组大鼠血清IL-1β、IL-6和TNF-α含量。结果厚朴酚抑制LPS处理的RAW264.7细胞中的NO、PGE2和IL-6的产生(P<0.05)。此外,厚朴酚抑制MIA诱导OA大鼠模型的IL-1β、TNF-α和IL-6的升高及MMP-2、MMP-9和COX-2的合成(P<0.05)。结论厚朴酚在OA大鼠模型中发挥有效的抗炎活性并保护软骨。

引用

页码：416 / 421

页数：6

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