流动注射-抑制化学发光法测定绿茶中的茶多酚含量

AIM To establish a flow injection chemiluminescence (CL) inhibition method for determination of tea polyphenols in green tea. METHODS The flow line of the sample, H 2O 2 and KIO 4 was inserted into luminol carrier solution. The mixed solution was led into the chemiluminescence cell for chemiluminescence reaction to occur and the CL intensity was recorded. RESULTS Tea polyphenols was shown to inhibit the CL reaction of luminol-H 2O 2-KIO 4 system in alkaline solution and the CL emission was inversely correlated with the tea polyphenols concentration. The linear range was 0.4～24.0 μg·mL -1 with a detection limit of 0.03 μg·mL -1 and a relative standard deviation of 1.3% (at tea polyphenols concentration 4.0 μg·mL -1, n=11). The sampling frequency was 240·h -1. CONCLUSION The method is simple, rapid and can be used for determination of tea polyphenols in green tea.