自发性高血压大鼠血浆代谢组学研究及人参总皂苷作用机制初探

被引：32

作者：

陆益红

王广基

黄青

阿基业

郝海平

郝琨

机构：

[1] 中国药科大学药物代谢与动力学研究中心

来源：

中国天然药物 | 2007年 / 06期

关键词：

自发性高血压大鼠; 代谢组学; GC/MS; 模式识别; 人参总皂苷;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

摘要：

目的:探讨自发性高血压大鼠(SHR)和正常大鼠(WKY)血浆代谢组差异,确证高血压模型,在此基础上探讨人参总皂苷对SHR血压及代谢组的影响。方法:15只12周龄的雄性SHR和15只同龄WKY大鼠经2周适应性饲养后测量大鼠尾动脉收缩压(SBP)、舒张压(DBP),同时收集血浆应用GC/MS测定内源性代谢物,用主成分分析(PCA)和偏最小二乘方判别分析(PLS-DA)分析两组动物的代谢谱及引起高血压的生物标记物。将SHR随机分成3组,人参总皂苷组:100mg.kg.d-1;卡托普利组:100mg.kg.d-1;SHR模型组:等量0.5%CMC-Na及WKY组等量0.5%CMC-Na,连续灌胃4周,SHR模型组和人参总皂苷组继续给药至8周后,测量大鼠尾动脉SBP、DBP及血浆内源性代谢物。结果:与WKY组相比,SHR组的SBP、DBP及血浆代谢组均有显著差异,SHR组内源性代谢物中十六酸、吡喃半乳糖、十八碳二烯酸、羟基丁酸等化合物的浓度显著增高。给药后,卡托普利组能显著降低SBP(P<0.01)和SBP(P<0.01);尽管人参总皂苷组对血压的作用不如卡托普利组明显,但前者的代谢谱较后者向正常组代谢谱靠近的趋势更加明显。结论:SHR组和WKY组血浆代谢组有显著差异,这将为进一步探讨高血压病相关的致病基因提供物质基础。长期应用人参治疗,能改善血浆代谢组,使机体的功能趋于正常。

引用

页码：443 / 447

页数：5

共 12 条

[1] Metabonomic classification of genetic variations in toxicological and metabolic responses using probabalistic neural networks. Holmes E,Nicholson JK,Tranter G. Chemical Research in Toxicology . 2001
[2] Rad ioprotective effect of Pa-nax ginseng on the phosphatases and lip id peroxidation level in testes ofSw iss alb ino m ice. KumarM,SharmaMK,Saxena PS,et al. B iol Pharm Bu ll . 2003
[3] The genetics of essential hypertension. Kevin M. O’Shaughnessy. British Journal of Clinical Pharmacology . 2001
[4] Metabonomics:a plat-formfor studying drugtoxicity and gene function. NicholsonJK,ConnellyJ,LindonJC,et al. Nature Reviews Drug Discovery . 2002
[5] Genetics of hypertension. Munroe PB,Caulfield MJ. Curr Opin Genet Dev . 2000
[6] Intra peritoneal injection of ginseng ex-tract enhances bothimmunoglobulin and cytokine productionin mice. Liou CJ,Li ML,Tseng J. American Journal of Chinese Medicine . 2004
[7] Ginseng:potential for the enhancement of cognitive performance and mood. Kennedy DO,Scholey AB. Pharmacol Biochem Behav . 2003
[8] Extraction and GC/MS analysis of the human blood plasma metabolome. Jiye A,Trygg J,Gullberg J,et al. Analytical Chemistry . 2005
[9] Changes in average blood pressure and incidence of high blood pressure 1983-1984 to 1987-1988 in four population cohorts in the People’s Republic of China. Wu XG,Huang ZD,Stamler J,et al. Journal of Hypertension . 1996
[10] Chemometric models for toxicity based on NMR spectra of biofluids. Holmes E,Nicholls AW,Lindon JC,et al. Chemical Research in Toxicology . 2000

← 1 2 →