利用pmi作为筛选基因对辣椒进行Bt基因转化的研究

被引：3

作者：

黄皓

雷建军

曹必好

陈长明

陈清华

陈国菊

机构：

[1] 华南农业大学园艺学院

来源：

辣椒杂志 | 2013年 / 11卷 / 02期

关键词：

辣椒; Cry2Aa2基因; 甘露糖; 遗传转化;

D O I：

10.16847/j.cnki.issn.1672-4542.2013.02.007

中图分类号：

S641.3 [辣椒];

学科分类号：

090202 ;

摘要：

根据Cry2Aa2的保守基因序列设计引物,从而扩增出完整的Cry2Aa2序列,连接到含有磷酸甘露糖异构酶基因(pmi)的表达载体pCAMBIA1301-PMI上,采用农杆菌介导的方法转化辣椒,利用甘露糖筛选体系对辣椒转化体进行筛选,对转基因植株进行分子生物学检测和抗虫试验,72h后统计抗虫情况。结果表明:获得了含有Cry2Aa2转基因植株;食用转Bt基因辣椒叶片和果实的斜纹夜蛾幼虫的校正死亡率均高于阴性对照,且大多表现出僵化和厌食的状态,对转基因的辣椒伤害较小,说明Bt基因成功导入辣椒中。

引用

页码：23 / 28

页数：6

共 9 条

[1]

Efficient biolistic transformation of maize（Zea mays L.）andwheat（Triticum aestivum L.）using the phosphomannose i-somerase gene,pmi,as the selectable marker. Wright M,Dawson J,Dunder E,Suttie J,Reed J,Kramer C,Chang Y,Novitzky R,Wang H,Artim-Moore L. Plant Cell Reports . 2001

[2] The use of the phosphomannose isomerase gene as alternative selectable marker for Agrobacterium-mediated transformation of flax (Linum usitatissimum) [J].

Lamblin, Frederic ;

Aime, Aurelie ;

Hano, Christophe ;

Roussy, Isabelle ;

Domon, Jean-Marc ;

Van Droogenbroeck, Bart ;

Laine, Eric .

PLANT CELL REPORTS, 2007, 26 (06) :765-772

[3] The use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants [J].

Degenhardt, Juliana ;

Poppe, Annika ;

Montag, Jurith ;

Szankowski, Iris .

PLANT CELL REPORTS, 2006, 25 (11) :1149-1156

[4]

The use of the PMI/mannose selection system to recover transgenic sweet orange plants ( Citrus sinensis L. Osbeck)[J] . &nbspPlant Cell Reports . 2003 (2)

[5]

Efficient biolistic transformation of maize ( Zea mays L.) and wheat ( Triticum aestivum L.) using the phosphomannose isomerase gene, pmi , as the selectable marker[J] . M. Wright,J. Dawson,E. Dunder,J. Suttie,J. Reed,C. Kramer,Y. Chang,R. Novitzky,H. Wang,L. Artim-Moore. &nbspPlant Cell Reports . 2001 (5)

[6]

Phosphomannose isomerase: An efficient selectable marker for plant transformation[J] . Janet Reed,Laubra Privalle,M. Luann Powell,Moez Meghji,John Dawson,Erik Dunder,Janet Sutthe,Allan Wenck,Karen Launis,Catherine Kramer,Yin-Fu Chang,Genevieve Hansen,Martha Wright. &nbspIn Vitro Cellular & Developmental Biology - Plant . 2001 (2)

[7]

Phosphomannose isomerase: A versatile selectable marker forArabidopsis thaliana germ-line transformation[J] . Rebecca Todd,Brian Tague. &nbspPlant Molecular Biology Reporter . 2001 (4)

[8]

Mannose Selection System in Tomato Transformation. Wang H W. Northeast Agricultural university . 2009

[9]

A new selection system for pepper regenration by mannos. Kim J Y,Jung M,Kin H S,Lee Y H,Choi S H,Lim Y P,Min B W,Yang S G,Ham C H. Jounal Plant Biotechnology . 2002

← 1 →