显微CT在骨微结构研究中的应用

被引：13

作者：

王维刚 ^{[1
,2
]}

刘震泽 ^{[2
]}

李俊 ^{[3
]}

严惠敏 ^{[3
]}

机构：

[1] 同济大学化学系

[2] 同济大学生命科学与技术学院

[3] 上海南方模式生物研究中心

来源：

中国细胞生物学学报 | 2012年 / 34卷 / 01期

关键词：

显微CT; 骨微结构; 骨质疏松; 骨保护素; 基因敲除小鼠;

D O I：

暂无

中图分类号：

R580 [];

学科分类号：

摘要：

该文对OPG基因敲除小鼠进行micro-CT扫描,进行全身骨骼三维重建,并分析股骨结构。结果发现OPG基因敲除纯合子小鼠全身骨骼出现弥漫性蜂窝状低密度影。OPG基因敲除小鼠的骨密度、骨结构模型指数、骨小梁厚度、骨体积分数、骨表面积与体积比、连接密度以及皮质骨厚度等指标均明显小于同窝野生型小鼠,而两基因型小鼠在骨小梁数目、骨小梁分离度及各项异性度等方面无明显差异。以上数据结果表明,OPG基因敲除导致小鼠出现骨质疏松。应用micro-CT可以对小鼠骨进行无创微结构分析,较体视学方法具有一定的优势。

引用

页码：16 / 22

页数：7

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