草鱼胰岛素样生长因子-Ⅰ基因克隆及序列分析

被引：10

作者：

白俊杰

叶星

李英华

李新辉

简清

罗建仁

机构：

[1] 中国水产科学研究院珠江水产研究所!广东广州

[2] 中国水产科学研

来源：

水产学报 | 2001年 / 01期

关键词：

草鱼; 胰岛素样生长因子I; 基因克隆;

D O I：

暂无

中图分类号：

Q785 [转化及克隆];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

采用逆转录 -聚合酶链式反应 (RT PCR)方法 ,从草鱼肝脏的总RNA中扩增出胰岛素样生长因子 I(IGF I)基因序列 ,定向克隆至质粒pUC18。测定了该基因序列 ,推导其编码的蛋白质序列。克隆的cDNA序列编码包括B、C、A、D和E五个区域的 117个氨基酸。与鲤IGF I成熟肽比较 ,核酸序列和氨基酸序列的同源性分别为 93.8%和 97.1%。E区域分析结果表明 ,所克隆的草鱼IGF I序列属于IGF IEa 2亚型

引用

页码：1 / 4

页数：4

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