[Object]To investigate the purification conditions of total flavonoids from Aurantii immaturus Fructus by macroporous resin.[Method] The adsorption quantity and desorption rate of three kinds of macroporous resins (AB-8,HPD-450,D101) were compared;the separation parameters of D101 on total flavonoids were studied.[Results] D101 is the best macroporous resin on the separation of total flavonoids from Fructus Aurantii immaturus.The optimal adsorption conditions are as follows:3 mg/ml of total flavonoids concentration,sample volume is 2 times of the bed volume (BV),pH 4.5,70% ethanol as eluent and elution volume is 2 times of BV.[Conclusion] D101 macroreticular resin showed better comprehensive adsorption property and can be used to separate and purify the total flavonoids in Aurantii immaturus Fructus.
