人髌下脂肪垫来源脂肪间充质干细胞的分离、培养及鉴定

被引：10

作者：

刘玉平 ^{[1
]}

刘涛 ^{[1
]}

王明明 ^{[1
]}

李明 ^{[1
]}

俞光荣 ^{[2
]}

机构：

[1] 滕州市中心人民医院创伤骨科

[2] 同济大学附属同济医院足踝外科

来源：

中国组织工程研究 | 2015年 / 19卷 / 41期

关键词：

干细胞; 间质干细胞; 细胞培养技术; 抗原,表面; 组织工程; 脂肪干细胞; 脂肪间充质干细胞; 细胞培养; 种子细胞; 表面抗原;

D O I：

暂无

中图分类号：

R329.2 [人体细胞学];

学科分类号：

100107 [人体解剖与组织胚胎学（人体解剖学、组织与胚胎学）];

摘要：

背景:髌下脂肪垫在膝关节手术中经常要部分切除,其可以作为脂肪间充质干细胞的重要来源。目的:探讨自髌下脂肪垫中分离、培养脂肪间充质干细胞的策略及细胞分子表面标记情况。方法:髌下脂肪垫组织取自膝关节镜手术的患者,以Ⅰ型胶原酶消化消化脂肪组织获取干细胞,用10%低糖DMEM培养基培养,利用MTT法测定不同代细胞增殖情况并绘制生长曲线。检测第5代细胞表面CD29及CD44的表达。结果与结论:培养24 h后可见原代细胞贴壁,1周后细胞呈纺锤型并且增殖速度加快,传代后的细胞贴壁及增殖细胞速度加快。生长曲线示第2及第5代的细胞增殖能力明显较第8代能力强。所取细胞能够分化为骨细胞和脂肪细胞。流式细胞仪检测结果显示第5代脂肪间充质干细胞重96.8%表达CD29,97.6%表达CD44。提示自髌下脂肪垫分离及提取脂肪干细胞简单易行,所得细胞的纯度及增殖能力均符合组织工程种子细胞的基本条件。

引用

页码：6566 / 6571

页数：6

共 12 条

[1]

Inhibition of Oxygen-Glucose Deprivation-Induced Apoptosis of Human Adipose-Derived Stem Cells by Genetic Modification with Antiapoptotic Protein Bcl-2 [J].

Cui, Ziwei ;

Shen, Liangyun ;

Lin, Yue ;

Wang, Shuqin ;

Zheng, Dongfeng ;

Tan, Qian .

AESTHETIC PLASTIC SURGERY, 2014, 38 (04) :779-787

[2]

Age-dependent impairment of number and angiogenic potential of adipose tissue-derived progenitor cells [J].

Madonna, Rosalinda ;

Renna, Francesca V. ;

Cellini, Carlo ;

Cotellese, Roberto ;

Picardi, Nicola ;

Francomano, Franco ;

Innocenti, Paolo ;

De Caterina, Raffaele .

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2011, 41 (02) :126-133

[3]

Chondrogenic potential of bone marrow- and adipose tissue-derived adult human mesenchymal stem cells [J].

Ronziere, M. C. ;

Perrier, E. ;

Mallein-Gerin, F. ;

Freyria, A. -M. .

BIO-MEDICAL MATERIALS AND ENGINEERING, 2010, 20 (3-4) :145-158

[4]

Isolation, identification and multipotential differentiation of mouse adipose tissue-derived stem cells [J].

Taha, Masoumeh Fakhr ;

Hedayati, Vahideh .

TISSUE & CELL, 2010, 42 (04) :211-216

[5]

Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.[J].Gang Yu;Xiying Wu;Marilyn A. Dietrich;Paula Polk;L. Keith Scott;Andrey A. Ptitsyn;Jeffrey M. Gimble.Cytotherapy.2010, 4

[6]

An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples [J].

Baptista, Leandra S. ;

do Amaral, Ronaldo J. F. C. ;

Carias, Rosana B. V. ;

Aniceto, Marcelo ;

Claudio-da-Silva, Cesar ;

Borojevic, Radovan .

CYTOTHERAPY, 2009, 11 (06) :706-715

[7]

Epidermal Growth Factor as a Candidate for Ex Vivo Expansion of Bone Marrow–Derived Mesenchymal Stem Cells.[J].KenichiTamama;Vivian H.Fan;Linda G.Griffith;Harry C.Blair;AlanWells.STEM CELLS.2009, 3

[8]

Basic Fibroblast Growth Factor Controls Migration in Human Mesenchymal Stem Cells.[J]..STEM CELLS.2009, 7

[9]

Concise Review: Adipose Tissue‐Derived Stromal Cells—Basic and Clinical Implications for Novel Cell‐Based Therapies.[J]..STEM CELLS.2009, 4

[10]

Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].

Dominici, M. ;

Le Blanc, K. ;

Mueller, I. ;

Slaper-Cortenbach, I. ;

Marini, F. C. ;

Krause, D. S. ;

Deans, R. J. ;

Keating, A. ;

Prockop, D. J. ;

Horwitz, E. M. .

CYTOTHERAPY, 2006, 8 (04) :315-317

← 1 2 →