人类生精相关基因TSARG4的cDNA克隆

被引：5

作者：

邢晓为

李麓芸

傅俊江

朱文兵

刘刚

刘上峰

卢光琇

机构：

[1] 中南大学湘雅医学院人类生殖工程研究室

来源：

生物化学与生物物理学报 | 2003年 / 03期

关键词：

基因克隆; 间隙填充法; 逆转录聚合酶反应; 睾丸; 组织表达谱;

D O I：

暂无

中图分类号：

Q987 [人类遗传学];

学科分类号：

071007 ;

摘要：

为了探索精子生成的分子机制 ,从人精子外部致密纤维蛋白相关基因SPAG4(spermantigen 4)和小鼠精母细胞中表达的AK0 0 62 2 5基因出发 ,找到两个人类EST ,BG72 0 5 64和AI70 0 45 4,其中BG72 0 5 64在人睾丸中表达。运用“间隙填充法”填平这两个EST之间的间隙 ,从人睾丸文库中快速克隆了同源于SPAG4和AK0 0 62 2 5基因的人类TSARG4基因 (testisandspermatogenesisrelatedgene 4) (GenBank登录号为AF40 13 5 0 ) ,并用RT PCR对该基因阅读框进行验证。TSARG4基因全长 12 5 2bp ,开放阅读框为 94～ 12 3 3bp ,定位于 2 0q11.2 ,推定编码 3 79个氨基酸 ,预计分子量为 43 0 81.45 ,等电点为 8.61,该基因与小鼠精母细胞基因AK0 0 62 2 5编码的氨基酸序列同源性 74% ,与人类SPAG4基因编码的氨基酸序列同源性 45 %。RT PCR表明人类TSARG4基因在多个组织中均有表达 ,而同源的小鼠AK0 0 62 2 5基因仅在睾丸中表达

引用

页码：283 / 288

页数：6

共 11 条

[1] Developmental Biology[C]. 2008年国际口腔及颅颌前沿研究研讨会,1600
[2] Sun C,Skaletsky H,Birren B,Devon K,Tang Z,Silber S,Oates R et al.An azoospermic man with a de novo point mutation in the Ychromosomal gene USP9 Y. Nature Genetics . 1999
[3] FU JJ,Li LY,Lu GX.Relationship between microdeletion on Y chromosome and patients with idiopathic azoospermia and severe oligozoospermia in the Chinese. Chinese Medical Journal . 2002
[4] Pruitt KD,Katz KS,Sicotte H,Maglott DR.Introducing RefSeq and LocusLink: Curated human genome resources at the NCBI. Trends in Genetics . 2000
[5] Kierszenbaum AL.Spermatid manchette: Plugging proteins to zero into the sperm tail. Molecular Reproduction and Development . 2001
[6] Tarnasky H,Gill D,Murthy S,Shao X,Demetrick DJ,van der Hoorn FA.A novel testis-specific gene, SPAG4, whose product interacts specifically with outer dense fiber protein ODF27, maps to human chromosome 20q11. 2. Cytogenetics and Cell Genetics . 1998
[7] Brown GM,Furlong RA,Sargent CA,Erickson RP,Longepied G,Mitchell M,Jones MH et al.Characterization of the coding sequence and fine mapping of the human DFFRY gene and comparative expression analysis and mapping to the Sxrb interval of the mouse Y chromosome of the Dffry gene. Human Molecular Genetics . 1998
[8] Burmester S,Hoyer-Fender S.Transcription and translation of the outer dense fiber gene ( Odf1 ) during spermiogenesis in the rat.A study by in situ analyses and polysome fractionation. Molecular Reproduction and Development . 1996
[9] Yen P.The fragility of fertility. Nature Genetics . 2001
[10] Altschul SF,Gish W,Miller W,Myers EW,Lipman DJ.Basic local alignment search tool. Journal of Molecular Biology . 1990

← 1 2 →