氧化型脂蛋白(a)对人脐静脉血管内皮细胞P选择素表达的影响

被引:1
作者
赵水平
许丹焰
机构
[1] 中南大学湘雅第二医院心内科,中南大学湘雅第二医院心内科
关键词
脂蛋白类; P选择素; 动脉粥样硬化;
D O I
暂无
中图分类号
R543.5 [动脉疾病];
学科分类号
摘要
目的 探讨氧化修饰的脂蛋白 (a) [ox Lp(a) ]能否诱导人脐静脉内皮细胞表达P选择素 ,以阐明其在动脉粥样硬化发生中的作用。方法 在内皮细胞培养基中分别加入天然脂蛋白 (a)[n Lp(a) ]与ox Lp(a)及天然与氧化的低密度脂蛋白 (n LDL、ox LDL)。用细胞酶联免疫检测P选择素蛋白的表达 ,用单核细胞黏附率试验检测单核细胞黏附 ,并用Northern杂交检测P选择素mRNA的表达。结果 ox Lp(a)呈剂量依赖性增加内皮细胞P选择素蛋白表达 ,10nmol/L及 2 0nmol/Lox Lp(a)分别使P选择素表达明显增加到 (145± 10 ) %及 (2 0 2± 2 2 ) %。同时观察到ox Lp(a)促使单核细胞黏附率增加 ,并且黏附率与加入的ox Lp(a)浓度呈正相关。四种脂蛋白的作用进行比较显示ox Lp(a)的作用最强 ,10nmol/Ln LDL、n Lp(a)、ox LDL、ox Lp(a)分别使P选择素表达增加到 (10 5± 7) %、(12 7± 9) %、(176± 19) %、(2 45± 2 2 ) %。Northernblot结果显示ox Lp(a)能促使P选择素mRNA表达明显增加。结论 ox Lp(a)能诱导内皮细胞表达P选择素增强 ,这可能与脂蛋白 (a)致动脉粥样硬化作用机制有关。
引用
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页码:44 / 48
页数:5
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  • [1] Levels of expression of Pselectin, E-selectin, and intercellular adhesion molecule-1 in coronary atherectomy specimens from patients with stable and unstable angina pectoris. Tenaglia AN,Buda AJ,Wilkins RG,et al. The American Journal of Cardiology . 1997
  • [2] Atherosclerosis[P]. DONG CHUNMING;GOLDSCHMIDT PASCAL J.中国专利:US2008095751A1,2008-04-24
  • [3] Structural and functional studies of the endothelial activation antigen endothelial leukocyte adhesion molecule-1 using a panel of monoclonal antibodies. Pigott R,Neodham LA,Edwards RM,et al. Journal of Immunology . 1991
  • [4] Lipoprotein ( a) and Atherosclerosis. Ishibashi S. Arteriosclerosis and Thrombosis . 2001
  • [5] Detection of new epitopes formed upon oxidation of low-density lipoprotein, lipoprotein(a) and very-lowdensity lipoprotein. Jurgens G,Ashy A,Esterbauer H. Biochemical Journal . 1990
  • [6] Impairment of endotheliumdependent dilation in rabbit renal arteries by oxidized lipoprotein(a): role of oxygen derived radicals. Galle J,Bengen J,Schollmeyer P,et al. Circulation . 1995
  • [7] The pathogenesis of atherosclerosis: a perspective for the 1990s. Ross R. Nature . 1993
  • [8] Relation between lipoprotein(a) and in vitro oxidation of apolipoprotein B-containing lipoproteins. Serdar Z,Sarandol E,Dirican M,et al. Clinical Biochemistry . 2000
  • [9] Induction of P-selectin by oxidized lipoprotein: separate effects on synthesis and surface expression. Vora DK,Fang ZT,Liva SM,et al. Circulation Research . 1997
  • [10] Oxidized low-density lipoprotein induces the expression of P-selectin ( GMP140/ PADGEM/CD 62) on human endothelial cells. Gebuhrer V,Murphy JF,Bordet JC,et al. Biochemical Journal . 1995