CpGDNA预防小鼠哮喘模型的形成

被引：9

作者：

金美玲

蔡映云

袁正宏

郑玲洁

张伟

机构：

[1] 复旦大学附属中山医院肺科

[2] 复旦大学医学院分子病毒实验室

[3] 复旦大学附属中山医院肺科上海

[4] 上海

来源：

复旦学报(医学版) | 2002年 / 05期

关键词：

含胞嘧啶鸟嘌呤核苷酸的免疫刺激元件; 变应性哮喘; 免疫调节;

D O I：

暂无

中图分类号：

R-332 [医用实验动物学];

学科分类号：

1001 ;

摘要：

目的　研究含胞嘧啶鸟嘌呤核苷酸的免疫刺激元件 (CpGDNA)能否预防卵蛋白致敏小鼠哮喘模型的形成。方法　将 18只BALB/c小鼠均分为 3组 ,其中卵蛋白致敏哮喘组 (OVA组 )和CpG预防组 (CpG组 )皮下注射卵蛋白致敏制作哮喘模型 ,然后用卵蛋白激发 2次 ;阴性对照组 (NS组 )皮下注射生理盐水 ,然后生理盐水激发 2次。CpG组在致敏前腹腔注射CpGDNA 10 0 μg/ 10 0 μL ,其他两组不作干预。 3组分别在第 2次激发后 2 4h测外周血OVA特异性IgE、IgG1、IgG2a,并处死小鼠测肺泡灌洗液 (BALF)中的细胞总数及嗜酸性粒细胞 (EOS)计数 ,肺组织病理切片观察形态学改变 ,并测定脾细胞培养上清液中OVA特异性IFN γ。结果　CpG组BALF中细胞总数和嗜酸性粒细胞明显低于OVA组 ,P <0 .0 5 ;CpG组肺组织炎症反应较OVA组明显减轻 ;CpG组脾细胞培养上清液中OVA特异性IFN γ的浓度明显高于OVA组 ,P <0 .0 5 ;CpG组外周血OVA特异性IgE和IgG1均低于OVA组 ,P <0 .0 5 ;CpG组IgG2a较OVA组为高 ,P <0 .0 5。结论　CpGDNA能预防卵蛋白致敏小鼠哮喘模型的形成

引用

页码：376 / 378

页数：3

共 12 条

[1]

Allergic and nonallergic asthmatics have distinct patterns of T-cell activation and cytokines production in peripheral blood and bronchoalveolar lavage. Walker C,Bode E,Boer L,et al. The American Review of Respiratory Disease . 1992

[2]

Prevalence of asthma and allergic disorders among children in united Germany: a descripitive comparison. von Mutius E,Fritzsch SK,Weiland G,et al. British Medical Journal . 1992

[3]

Modulation of airway inflammation by CpG oligodeoxynucleotides in a murine model of asthma. Kline JN,Waldschmidt TJ,Businga TR,et al. J Immunol . 1998

[4]

Sorting out the cytokines of asthma. Drazen JM,Arm JP,Austen KF,et al. The Journal of Experimental Medicine . 1996

[5]

Preferential induction of a Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization. Raz E,Tighe H,Sato Y,et al. Proceedings of the National Academy of Sciences of the United States of America . 1996

[6]

Allergy[P]. 英国专利:GB202319091D0,2024-01-31

[7]

Immunostimulatory DNA sequences necessary for effective intradermal gene immunization. Sato Y,Roman M,Tighe H,et al. Science . 1996

[8]

Immunostimulatory DNA sequences inhibit IL 5, eosinophilic inflammation, and airway hyperres-ponsiveness in mice. Broide D,Schwarze J,Tighe H,et al. J Immunol . 1998

[9]

DNA-based immunization for asthma. Broide D,Raz E. International Archives of Allergy and Immunology . 1999

[10]

Interleukin 4, but not interleukin 5 or eosinophils, is required in a murine model of acute airway hyperreactivity. Corry D,Folkesson HG,Warnock M,et al. The Journal of Experimental Medicine . 1996

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