基于酪氨酸酶抑制作用的莠去津农药残留电化学快速检测

被引：4

作者：

韩恩

潘超

曹晓梅

蔡健荣

机构：

[1] 江苏大学食品与生物工程学院

来源：

食品科技 | 2015年 / 40卷 / 05期

关键词：

电化学生物传感; 酪氨酸酶; 莠去津农药; 快速检测;

D O I：

10.13684/j.cnki.spkj.2015.05.070

中图分类号：

O657.1 [电化学分析法]; TS207.53 [];

学科分类号：

摘要：

基于莠去津农药对酪氨酸酶的抑制作用,采用聚二烯丙基二甲基氯化铵(PDDA)功能化的碳纳米管在玻碳电极表面固载酪氨酸酶,构建了一种能够快速检测莠去津农药残留的电化学生物传感器。试验优化了酶电极制作的厚度以及莠去津农药对酪氨酸酶的抑制时间,结果表明,该电化学生物传感器对莠去津农药检测的线性范围为0.01～0.8μg/m L,检测限低至4 ng/m L。该电化学检测方法操作简单、检测速度快、灵敏度高,可用于实际样品中莠去津农药残留检测。

引用

页码：344 / 347

页数：4

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