纤溶酶-α2-抗纤溶酶复合物单克隆抗体的制备及其应用

被引：3

作者：

王红

李稻

李建新

王鸿利

机构：

[1] 上海第二医科大学医学检验重点实验室

[2] 上海第二医科大学医学检验重点实验室（）

[3] （）

来源：

诊断学理论与实践 | 2002年 / 01期

关键词：

纤溶酶-α2-抗纤溶酶复合物; 单克隆抗体; 双抗体夹心法ELISA;

D O I：

暂无

中图分类号：

R446.62 [免疫物质测定];

学科分类号：

100208 ;

摘要：

目的:制备纤溶酶-α2-抗纤溶酶复合物（plasmin-α2-antiplasmin complex,PAP）的单克隆抗体并建立其酶免疫检测方法。方法:从人血浆中提纯的PAP作为免疫原制备特异性单克隆抗体,通过双抗体夹心法酶联免疫吸附试验（ELISA）对该单克隆抗体应用价值进行评估。结果:获得特异针对PAP新抗原的单抗3C10和针对PAP及纤溶酶原（Plg）的单抗2B9,效价均为1.25X10-6,其对PAP的亲和常数分别为4.69x10-10M和5.62X10-9M。以此建立的双抗体夹心ELISA检测PAP在0～160ng/ml范围内呈良好的线性关系,批内CV<7％,检测敏感度为5ng/ml,与AD公司的PAP试剂盒相关性良好（r=0.9629）。结论:本法可应用于临床评估纤溶系统的激活状态。

引用

页码：39 / 42

页数：4

共 6 条

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