银杏叶提取物诱导骨髓间充质干细胞成骨分化过程中P38信号通路的研究

被引：5

作者：

时舒曼 ^{[1
]}

袁浩天 ^{[2
]}

张晓晓 ^{[1
]}

吴哲 ^{[1
,3
]}

机构：

[1] 吉林大学口腔医学院修复科

[2] 中国人民解放军医院

[3] 广州医科大学附属口腔医院

来源：

口腔医学研究 | 2016年 / 32卷 / 09期

关键词：

银杏叶提取物; 骨髓间充质干细胞; P38MAPK; 信号通路; 成骨分化;

D O I：

10.13701/j.cnki.kqyxyj.2016.09.005

中图分类号：

R580 [];

学科分类号：

1002 ; 100201 ;

摘要：

目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)促进大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的机制。方法:将150mg/L GBE作用于第3代大鼠BMSCs,抑制组使用P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路抑制剂SB203580预处理,通过茜素红(alizarin red S,ARS)染色、碱性磷酸酶(Alkalinet phosphatase,ALP)法以及RT-PCR等检测方法判定GBE是否通过上调P38信号通路促进大鼠BMSCs成骨分化。结果:与实验对照组大鼠相比,抑制组的大鼠BMSCs细胞外基质矿化减少,ALP表达明显下降,成骨相关基因ALP、BMP-2、OCN、Runx2(runt-related transcription factor2)的表达量亦明显降低。结论:GBE通过上调P38信号通路促进大鼠BMSCs成骨分化。

引用

页码：912 / 915

页数：4

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