白芍总苷对糖尿病大鼠肝脏内质网应激的调节作用

被引：5

作者：

夏玲玲

周仲松

谢琴秀

朱启金

机构：

[1] 安徽医科大学第一附属医院感染病科

来源：

安徽医科大学学报 | 2015年 / 50卷 / 10期

基金：

安徽省自然科学基金;

关键词：

糖尿病; 肝脏; 内质网应激; 炎症; 白芍总苷;

D O I：

10.19405/j.cnki.issn1000-1492.2015.10.020

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的探讨白芍总苷(TGP)对糖尿病大鼠肝脏损害的保护作用是否与抑制内质网应激(ERS)有关。方法建立链脲佐菌素(STZ)诱导的糖尿病模型,随机分为对照组、模型组、TGP(50、100、200 mg/kg)给药组。应用油红O与Masson染色对肝组织行病理检查;采用免疫组化法检测肝组织ED-1表达;采用Western blot法检测GRP78、p-Perk及p-Eif2α的表达。结果 TGP给药可降低糖尿病大鼠肝重增加及肝组织总胆固醇(TC)、三酰甘油(TG)及游离脂肪酸(FFA)水平。模型组肝细胞油红O染色评分明显高于对照组(P<0.01);TGP各给药组评分明显低于模型组(P<0.01)。Masson染色模型组肝纤维化评分明显高于对照组(P<0.01);TGP 50、100、200 mg/kg给药组评分明显低于模型组(P<0.01)。免疫组化显示模型组肝组织巨噬细胞浸润明显增加,各给药组均能抑制糖尿病肝组织巨噬细胞浸润的增加。Western blot显示糖尿病模型组肝组织GRP78、pPerk及p-Eif2α表达明显高于对照组,TGP给药组肝组织GRP78、p-Perk及p-Eif2α表达明显低于模型组。结论 TGP对糖尿病肝损害有明显保护作用,其机制可能与其抗炎、抑制ERS有关。

引用

页码：1451 / 1455

页数：5

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