广西眼镜蛇毒磷酸二酯酶分离纯化及部分性质

被引：6

作者：

韦世秀

舒雨雁

黎红烨

机构：

[1] 广西医科大学医学科学实验中心,广西医科大学蛇毒研究所,广西医科大学蛇毒研究所南宁,南宁,南宁

来源：

中国生物化学与分子生物学报 | 2005年 / 02期

关键词：

cobra venom; phosphodiesterase; purification & identification; acute toxicity; analgesia;

D O I：

10.13865/j.cnki.cjbmb.2005.02.028

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

By successive chromatography on CM-Sepharose CL-6B,Sephadex G-100,Heparin-Sepharose CL-6B, a phosphodiesterase (PDE, EC3.1.4.1) was isolated and purified from Guangxi cobra venom. The final preparation was 89 fold pure and had high specific activity. The enzyme showed a single band on SDS-PAGE and IEF. The PDE showed a molecular weight of 110 kD and pI of 7.4. The enzyme consists of 840 amino acid residues and has only a methionine as N-terminal amino acid residues. It was rich in asparagine, leucine and glutamine and poor in sulphur-containing amino acid. It contains 5.38% neutral sugars. It has no acute toxicity and has analgesia effect.

引用

页码：277 / 280

页数：4

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