大鼠骨髓基质细胞体外定向诱导成骨

被引：8

作者：

陈克明

葛宝丰

刘兴炎

白孟海

郑荣梁

机构：

[1] 兰州大学生命科学学院

[2] 兰州军区兰州总医院骨科研究所

[3] 兰州大学生命科学学院甘肃兰州

[4] 甘肃兰州

来源：

兰州大学学报 | 2003年 / 01期

关键词：

骨髓基质细胞; 分化; 诱导; 成骨细胞;

D O I：

10.13885/j.issn.0455-2059.2003.01.015

中图分类号：

R329 [人体组织学];

学科分类号：

100101 ;

摘要：

将大鼠骨髓单细胞悬液静置培养 36h,利用骨髓基质细胞贴壁能力强的特点对其进行纯化和扩增培养 .采用爬片培养、HE染色、组化染色以及碱性磷酸酶活性和钙含量测定等手段研究培养骨髓基质细胞的形态、分化和分泌基质情况 .结果表明 ,非诱导培养条件下骨髓基质细胞呈梭形 ,部分传代细胞中可观察到脂肪细胞和肌细胞 .经成骨性诱导培养后 ,骨髓基质细胞发生明显的形态学变化 ,碱性磷酸酶活性上升 ,钙含量增加 ,最终形成典型的矿化结节 .提示培养大鼠骨髓基质细胞具有分化成脂肪细胞和肌细胞的能力 ,但其分化成骨的潜能最为强大 .本实验诱导骨髓基质细胞分化为成骨细胞的模式有可能适用于骨组织工程研究 .

引用

页码：69 / 73

页数：5

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