强光光抑制引起的光系统Ⅱ膜复合物蛋白二级结构的变化

被引：7

作者：

施桦

杨昆云

唐崇钦

匡廷云

赵南明

机构：

[1] 清华大学生物科学与技术系,中国科学院植物研究所,中国科学院植物研究所,中国科学院植物研究所,清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室,北京,北京,北京,北京,生物膜与膜生物工程国家重点实验室,北京

来源：

自然科学进展 | 1997年 / 04期

关键词：

光系统Ⅱ; 光抑制; 二级结构; 红外光谱;

D O I：

暂无

中图分类号：

Q945 [植物生理学];

学科分类号：

0903 ;

摘要：

强光照射引起的光系统Ⅱ膜蛋白复合物的光抑制过程中,伴随着二级结构组成发生变化:短时间的光照(<10 min)对其二级结构的影响不大,但较长时间(40min)后可引起α螺旋含量下降,无规卷曲成分增加.在复合物中加入表面活性剂TritonX-100后,可使β折叠的含量显著减少;同时还使PSⅡ对强光的耐受性下降,表现在二级结构的变化加大.实验结果表明:稳定的膜结构可以提高光系统Ⅱ抵抗强光破坏的能力,光抑制主要影响膜蛋白复合物的螺旋构象,而对折叠和转角影响不大。推测光破坏的主要位点发生于膜蛋白的疏水区域。

引用

页码：67 / 72

页数：6

共 9 条

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