简单重复序列区间(ISSR)引物反应条件优化与筛选

被引：144

作者：

余艳

陈海山

葛学军

机构：

[1] 中国科学院华南植物研究所

来源：

热带亚热带植物学报 | 2003年 / 01期

关键词：

ISSR; 引物筛选; 沙冬青;

D O I：

暂无

中图分类号：

Q943 [植物细胞遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

以豆科沙冬青(Ammopiptanthusmongolicus)的基因组DNA制备ISSR-PCR模板。通过对聚合酶链式反应(PCR)体系中模板DNA浓度、Mg2+浓度、dNTP的用量、Taq酶的含量以及退火温度梯度进行试验,探讨筛选出清晰、多态性高、可重复性好的ISSR引物的PCR反应条件。用100个ISSR引物进行了PCR扩增,筛选出效果较好的15个引物,得到121个位点,其中43个多态位点,多态位点比例为36%。

引用

页码：15 / 19

页数：5

共 14 条

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