黄芪多糖对肝癌HepG2细胞的抑制作用及其机制

被引：25

作者：

冯涛 ^{[1
]}

张保国 ^{[2
]}

机构：

[1] 南京市市级机关医院药剂科

[2] 南京医科大学附属南京第一医院肿瘤科

来源：

实用老年医学 | 2010年 / 24卷 / 06期

关键词：

黄芪多糖; 肝癌HepG2细胞株; 细胞增殖; 细胞凋亡;

D O I：

暂无

中图分类号：

R735.7 [肝肿瘤];

学科分类号：

摘要：

目的研究黄芪多糖(APS)和5-氟尿嘧啶(5-fu)联合使用对肝癌HepG2细胞的生长抑制及其作用机制。方法用RPMI1640培养液对低分化人肝癌细胞株HepG2进行传代培养,取对数生长期细胞,分为5组:A组:对照组,不含药物,使用同体积的磷酸盐缓冲液(PBS);B组:APS组,只给予100μg/mlAPS;C组:5-fu组,只给予100μg/ml5-fu;D组:5-fu+低浓度APS组,给予100μg/ml5-fu+100μg/mlAPS;E组:5-fu+高浓度APS组,给予100μg/ml5-fu+200μg/mlAPS。用噻唑蓝(MTT)法观察APS及5-fu对HepG2细胞增殖的影响;用流式细胞术方法测药物作用后细胞周期变化和细胞凋亡;western-blot检测凋亡相关蛋白表达。结果 APS和5-fu联合使用可抑制HepG2细胞的增殖,效应呈浓度和时间依赖性;APS和5-fu联合使用阻滞HepG2细胞周期于G1期;并可诱导HepG2细胞凋亡;APS和5-fu联合使用,可明显增高肝癌HepG2细胞中caspase-3、caspase-9蛋白的表达,同时检测到抑凋亡蛋白Bcl-2表达显著降低。在只使用5-fu的实验组细胞中均未检测到上述变化。结论 APS和5-fu联合使用能抑制肝癌HepG2细胞株的增殖,其机制之一是影响细胞周期使之阻滞于G1期,激活了细胞凋亡系统,从而诱导细胞凋亡。

引用

页码：486 / 488+495 +495

页数：4

共 10 条

[1] 老年肝癌的诊治进展 [J].

杨秉辉 ;

任正刚 .

实用老年医学, 2001, (03) :123-124

[2]

Bcl-2 cleavages at two adjacent sites by different caspases promote cisplatin-induced apoptosis[J]. Jianbei Zhu1;Ying Yang1;Jiarui Wu1,2 1 Kev Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China 2 Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science & Technology of China, Hefei 230027, China.Cell Rese

[3] 老年肝癌的诊治进展 [J].

周信达 ;

汤钊猷 .

中华老年多器官疾病杂志, 2005, (03) :164-165

[4]

Astragalus mongholicus polysaccharide inhibits lipopolysaccharide-induced production of TNF-α and interleukin-8[J]. Yuan Yuan, Mei Sun, Department of Pediatrics, Shengjing Affiliated Hospital of China Medical University, Shenyang 110004, Liaoning Province, China Yuan Yuan, Department of Pediatrics, Chinese PLA 211 Hospital, Harbin 150086, Heilongjiang Province, China Ke-Shen Li, Institute of Biomedical Engineering, Harbin Engineering University, Harbin 150001, Heilongjiang Province, China.World

[5] 正交实验优选黄芪多糖硫酸化修饰条件及修饰产物抗IBDV活性测定 [J].

黄小燕 ;

胡元亮 ;

卢宇 ;

张帆 ;

郭振环 .

中药材, 2008, (04) :588-592

[6]

Antimony-trioxide- and arsenic-trioxide-induced apoptosis in myelogenic and lymphatic cell lines, recruitment of caspases, and loss of mitochondrial membrane potential are enhanced by modulators of the cellular glutathione redox system[J] . Annals of Hematology . 2009 (11)

[7]

Caspases activation in hyperthermia-induced stimulation of TRAIL apoptosis[J] . Maryline Moulin,André-Patrick Arrigo.Cell Stress and Chaperones . 2008 (3)

[8] Merit of Astragalus polysaccharide in the improvement of early diabetic nephropathy with an effect on mRNA expressions of NF-κB and IκB in renal cortex of streptozotoxin-induced diabetic rats [J].

Zhang, Ying-Wen ;

Wu, Chao-Yan ;

Cheng, Hei-Tang .

JOURNAL OF ETHNOPHARMACOLOGY, 2007, 114 (03) :387-392

[9]

Macrophage activation by polysaccharide isolated from Astragalus membranaceus[J] . Kun Yeong Lee,Young Jin Jeon.International Immunopharmacology . 2005 (7)

[10]

Corncobinduced endo-1,4-β-D-xylanase of Aspergillus oryzae MTCC 5154: Production and characterization of xylobiose from glucuronoxylan .2 A. A. Aachary,S. G. Prapulla. J. Agric. Food Chem . 2008

← 1 →