黄芪多糖对毒胡萝卜素诱导大鼠心肌细胞内质网应激的影响

被引：9

作者：

袁成代

陈永波

机构：

[1] 宝鸡市中医医院药剂科

来源：

临床和实验医学杂志 | 2015年 / 14卷 / 16期

关键词：

大鼠; 黄芪多糖; 毒胡萝卜素; 心肌细胞; GRP78; CHOP;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

目的探讨黄芪多糖(APS)对毒胡萝卜素(Tg)作用下大鼠心肌细胞损伤以及内质网应激相关分子GRP78和CHOP的影响。方法原代培养SD大鼠乳鼠心肌细胞随机分为对照组、Tg组和Tg+APS组。对照组:单纯培养基不给予任何药物处理;Tg组:培养液中加Tg;Tg+APS组:培养液中加Tg和APS;APS组:培养基中仅加入APS。MTT法检测心肌细胞存活率,实时定量PCR法检测GRP78和CHOP表达水平。结果与对照组比较,Tg处理48 h心肌细胞存活率降低,损伤较明显;与Tg组比较,APS处理增加心肌细胞存活率,减少心肌细胞损伤。与对照组比较,Tg组GRP78和CHOP表达水平显著升高;与Tg组比较,Tg+APS组GRP78和CHOP表达水平显著下调。结论黄芪多糖能够抑制Tg诱导的心肌细胞损伤,其机制可能与拮抗GRP78和CHOP的表达、减轻内质网应激有关。

引用

页码：1323 / 1325

页数：3

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