新生鼠缺氧缺血性脑损伤后内源性神经干细胞的增殖及迁移

被引：17

作者：

屈素清

栾佐

闫凤青

尹国才

郭万里

胡晓红

吴南海

机构：

[1] 北京海军总医院儿科

来源：

中华小儿外科杂志 | 2005年 / 05期

关键词：

脑损伤; 干细胞; 动物,实验;

D O I：

暂无

中图分类号：

R722.12 [新生儿窒息];

学科分类号：

摘要：

目的观察新生鼠缺氧缺血损伤后内源性神经干细胞的增殖及迁移。方法制成新生鼠缺氧缺血脑损伤模型,采用单、双标免疫组织化学方法检测5溴脱氧尿苷(BrdU)和巢蛋白(Nestin)的表达。BrdU标记增殖的新生细胞,Nestin标记神经干细胞。结果正常新生鼠生后保持较短一段时间的增殖活跃,生后3d达高峰,7d后逐渐减弱,生后21d后形成与成年动物相似的发生模式。缺氧缺血损伤后,细胞增殖恢复活跃,BrdU阳性细胞数于伤后4d达高峰,皮层、纹状体、海马可见散在阳性细胞,大多数BrdU阳性细胞集中位于室管膜下区的背外侧角及外侧壁处,伤后7d后逐渐减少,21d更多的BrdU阳性细胞迁移至损伤区;损伤后Nestin阳性细胞数增加,同时一定数量的Nestin阳性细胞由G0期进入增殖周期。结论缺氧缺血脑损伤可刺激新生鼠内源性神经干细胞的增殖及迁移。

引用

页码：260 / 263

页数：4

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