异硫氰酸荧光素标记尿激酶的制备与性能研究

被引：3

作者：

张雅娟 ^{[1
]}

俞洁 ^{[1
]}

张冬未 ^{[1
]}

梁洪泽 ^{[1
]}

梁明明 ^{[2
]}

赵玲玲 ^{[1
]}

机构：

[1] 宁波大学材料科学与化学工程学院

[2] 马鞍山市人民医院

来源：

宁波大学学报(理工版) | 2016年 / 29卷 / 03期

基金：

浙江省自然科学基金;

关键词：

异硫氰酸荧光素; 荧光标记; 尿激酶; 溶栓;

D O I：

暂无

中图分类号：

R943 [制剂学];

学科分类号：

摘要：

用异硫氰酸荧光素（FITC）对溶栓蛋白药物尿激酶（uPA）进行了荧光标记,制备了荧光素标记的尿激酶（FITC-u PA）,经核磁谱及红外谱表征证实了反应能有效进行,其荧光素/蛋白值（F/P）值为0.45.尿激酶经荧光素标记后,具有良好的荧光性质,其荧光检测下限低达10-6 g?m L-1.在1～100mg?m L-1范围内,荧光强度与溶液浓度具有很好的线性关系,可用于微/痕量蛋白质的定量/定性检测和跟踪标记.荧光素标记后的尿激酶,其流体力学直径与未标记的尿激酶基本一致,其体外溶栓能力也与未标记的尿激酶相当,说明FITC标记基本不影响尿激酶的生物活性,是一种简单、有效的溶栓药物标记方法.

引用

页码：78 / 82

页数：5

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