谷氨酸影响星形胶质细胞表达神经生长因子的实验研究

被引：1

作者：

韦良臣

于涯涛

钟世镇

机构：

[1] 解放军驻香港部队医院外科

[2] 第一军医大学生物力学实验室!广州

来源：

神经解剖学杂志 | 1999年 / 04期

关键词：

神经生长因子; 星形胶质细胞; 谷氨酸; 乳鼠;

D O I：

暂无

中图分类号：

R33-33 [生理学实验和技术];

学科分类号：

摘要：

本文利用双向ＥＬＩＳＡ法定量检测体外培养乳鼠大脑皮质星形胶质细胞在不同浓度的谷氨酸条件培养基中ＮＧＦ的表达分泌量，观察谷氨酸的调节作用，并利用氯胺酮或ＥＧＴＡ限制谷氨酸ＮＭＤＡ型受体介导的钙离子内流，观察谷氨酸调节作用的改变。结果表明：体外培养的星状胶质细胞２４ｈ能表达分泌ＮＧＦ３．１２３ｐｇ／１０５细胞；１０ μｍｏｌ／Ｌ～１ｍｍｏｌ／Ｌ的谷氨酸能显著促进ＮＧＦ表达，其中以１００ μｍｏｌ／Ｌ的谷氨酸作用为最强，而１０ｍｍｏｌ／Ｌ的谷氨酸反而起抑制作用；限制谷氨酸ＮＭＤＡ型受体介导的钙离子内流，能消除谷氨酸的这种调节作用。结果提示：谷氨酸可因其浓度不同促进或抑制星形胶质细胞表达ＮＧＦ，这种调节作用可能通过其ＮＭＤＡ受体介导的钙离子内流来实现。

引用

页码：366 / 368

页数：3

共 9 条

[1] Neurotrophines and brain insults. Lindvall O,Kokaia Z,Bengzon J,et al. Transport for Initiating and Negotiating Sessions . 1994
[2] An enzyme-linked immunoassay for nerve growth factor ( NGF ): a tool for studying regulatory mechanism involved in NGF production in brain and in peripheral tissues. Weskamp G,Otten U. Journal of Neurochemistry . 1987
[3] Synthesis and secretion of nerve growth factor by mouse astroglia cells in culture. Furukawa S,Furukawa Y,Satoyoshi E,et al. Biochemical and Biophysical Research Communications . 1986
[4] I n vivo activated brain astrocytes may produce and secrete nerve growth factor-like molecules. Oderfeld-Nowak B,Bacia A,Gradkowska M,et al. Neurochemistry International . 1992
[5] Multiple conductancechannels in type-2 cerebellar astrocytes activated by excitatoryam ino acids. Usow icz M M,Gallo V,Cull-Candy SG. Nature . 1989
[6] Glutamate induces calcium waves in cultured astrocytes: long-range glial signalling. Cornell -Bell AH,Finkbeiner SM,Cooper MS,et al. Science . 1990
[7] Kainic acid-induced seizures stimulate increased expression of nerve growth factor mRNA in rat hippocampus. Gall CH. Molecular Brain Research . 1991
[8] Tropic factor production by reactive astrocytes in the injured brain. Schwartz J. Annals of the New York Academy of Sciences . 1994
[9] Regional specific and rapid increases in brain-derived neurotrophic factor messenger RNA in the adult rat brain following seizures induced by systemic administration of kainic acid. Dugich-Djordjevic MM,Tocco G,Lapchak PA,et al. Neuroscience . 1992

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