差异显示反转录PCR技术研究进展

被引:14
作者
赵锦荣
阎小君
苏成芝
机构
[1] 第四军医大学基因诊断技术应用研究所!西安
关键词
mRNA; 差异显示; 聚合酶链反应;
D O I
暂无
中图分类号
Q751 [];
学科分类号
摘要
分析一对细胞或组织在不同状态下基因表达的差异,已成为分子生物学研究领域的热点之一.近年来,用于识别差异表达基因的方法已发展起来多种.DDRTPCR是近年来较为广泛应用的一种技术.理论上,DDRTPCR技术比较简单,但实施起来却存在着假阳性率高,凝胶中单条cDNA带成分不均一,所获cDNA仅代表着mRNA3′UT区(约300bp)以及一些低拷贝数mRNA不能有效被呈现等问题.对DDRTPCR技术的改良也主要集中在解决这些问题方面.
引用
收藏
页码:27 / 31
页数:5
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