SLE患者外周血中RORγt的检测及其临床意义

被引：1

作者：

曹慧 ^{[1
]}

赵凯 ^{[1
]}

曹正欣 ^{[2
]}

机构：

[1] 金坛市人民医院检验科

[2] 江苏大学附属医院检验科

来源：

徐州医学院学报 | 2012年 / 11期

关键词：

系统性红斑狼疮; Th17细胞; RORγt; 白细胞介素-17;

D O I：

暂无

中图分类号：

R593.241 [];

学科分类号：

摘要：

目的检测系统性红斑狼疮(SLE)患者外周血中Th17细胞特异性转录因子及其相关细胞因子的表达水平,初步探讨Th17细胞在SLE发生发展中可能的作用。方法收集SLE患者(SLE组,n=41)和健康体检者(健康对照组,n=32)外周血标本,采用实时荧光定量PCR(QRT-PCR)检测外周血单个核细胞(PBMC)中维甲酸相关孤核受体γt (RORγt)和白细胞介素-17(IL-17)的mRNA水平;酶联免疫吸附试验(ELISA)检测血浆中IL-17、IL-23的蛋白浓度。结果 SLE活动期组PBMC中RORγt mRNA、IL-17 mRNA表达水平均明显高于SLE静止期组和健康对照组(P <0.01),血浆IL-17蛋白、IL-23蛋白表达水平也明显高于SLE静止期组和健康对照组(P<0.01)。结论 SLE患者外周血中存在较高水平的Th17细胞特异性转录因子和相关细胞因子。Th17细胞可能参与了SLE发病的病理生理过程,检测外周血Th17细胞特异性转录因子RORγt水平有助于反映SLE的活动性。

引用

页码：755 / 757

页数：3

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