转基因番茄防龋疫苗的基础研究 Ⅰ.变形链球菌pac基因唾液粘附区植物表达质粒的构建

被引：11

作者：

蒋少云

凌均启

机构：

[1] 中山医科大学口腔医学院!广东广州,中山医科大学口腔医学院!广东广州

来源：

口腔医学纵横 | 2000年 / 02期

关键词：

植物表达质粒; 表面蛋白基因; 唾液粘附区;

D O I：

10.13701/j.cnki.kqyxyj.2000.02.005

中图分类号：

R392-33 [免疫学技术、设备及实验方法];

学科分类号：

摘要：

目的 :构建变形链球菌表面蛋白基因 (pac)唾液粘附区的植物表达质粒pROP1和pRPB1。方法 :用PCR扩增的含变形链球菌表面蛋白唾液粘附区P1片段 (184- 1946bp)与植物的高效表达质粒pROKCP结合 ,构建pROP1表达质粒 ;且将此质粒与Bar基因 (抗除草剂基因 )连接 ,构建表达质粒pRPB1,酶切电泳检测。结果 :从pPC41中扩增的P1片段整合到pROKCP的适当部位 ,构成重组质粒pROP1,从pPBar分离出的Bar基因整合到pROP1,构成重组质粒pRPB1。结论 :本实验成功地构建了携带变形链球菌表面蛋白唾液粘附区的植物表达质粒pROP1和pRPB1。

引用

页码：94 / 96

页数：3

共 12 条

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