A Sensitive HPLC Technique for the Quantitation of Dauricine

被引:1
作者
陈淑娟
杨毅梅
张力
庞雪冰
代宗顺
曾繁典
机构
关键词
dauricine; RP-HPLC; concentration determination;
D O I
暂无
中图分类号
R-331 [实验方法与设备];
学科分类号
1001 ;
摘要
Summary: To establish the determination method of dauricine (Dan) concentration in rats’ blood and other biological samples, a reverse-phase HPLC method was adopted. Under the given condition, dauricine could be well separated. The retention time (tR) of Dan and its internal standard, daurisoline were 9. 2 and 6.1 respectively. The detection limit was 10-2 mg/ml. The absolute recoveries of all kinds of samples were above 70 %, and the relative ones were over 85%. A good liner relationship has been obtained over the entire range of 0. 030 to 3. 000 rug/L in blood samples and 0. 050 to 5. 000 mg/L in other tissue samples. The intraday and interday coefficients of varia- tion were below 10 %. The results showed that the method can be used for detecting Dan in all kinds of biological samples.
引用
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页码:39 / 42
页数:4
相关论文
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[1]  
Effects of dauricine,quinidine, and sotlol on action potential duration ofpapillary muscles in vitro. Guo D L, Zeng F D, Hu C J. Acta Pharmacological Sinica . 1997