抗赭曲霉毒素A单克隆杂交瘤细胞系的建立及特性

被引：5

作者：

阳传和

罗雪云

李业鹏

刘畅

机构：

[1] 卫生部食品卫生监督检验所

[2] 卫生部食品卫生监督检验所北京

[3] 北京

[4] 北京

来源：

细胞与分子免疫学杂志 | 1992年 / 02期

关键词：

赭曲霉毒素A; 抗体; 单克隆; ELISA;

D O I：

10.13423/j.cnki.cjcmi.000042

中图分类号：

学科分类号：

摘要：

用杂交瘤技术制备了4株稳定产生抗赭曲霉毒素A单克隆抗体的杂交瘤细胞株,命名为1A9、5F2、5G5和6G11。1A9、5G5和6G11的Ig亚类为IgG1,5F2的亚类为IgG2a。抗体腹水效价为500000～1000000。6G11检测纯毒素的线性范围为2～500ng/ml,最低检出量为1ng/ml。交叉反应的结果还表明,该单抗与共试的其他结构类似物无反应,具有较高的特异性。

引用

页码：17 / 21

页数：5

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