孝顺竹RNA提取方法研究

被引：18

作者：

杨卫东

王敬文

张金萍

费学谦

机构：

[1] 中国林业科学研究院亚热带林业研究所

来源：

林业科学研究 | 2005年 / 06期

基金：

浙江省自然科学基金;

关键词：

孝顺竹; RNA; 提取; 改良SDS法;

D O I：

10.13275/j.cnki.lykxyj.2005.06.023

中图分类号：

S795.9 [其他竹类];

学科分类号：

摘要：

<正>提取纯度高、完整性好、不含DNA或其它杂质的总RNA是进行Northern杂交、cDNA合成、cDNA 文库构建、RT-PCR及mRNA差异显示等分子生物学研究的基础。目前提取植物RNA方法有多种,这些方法设计旨在RNA提取过程中清除多糖、多酚等杂质污染。在提取高质量RNA的过程中,植物尤其木本植物通常含有大量的酚类、多糖和其他一些水溶性化合物与RNA共沉淀,影响了RNA功能。

引用

收藏

页码：769 / 772

页数：4

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