丙酰紫草素对烟草花叶病毒外壳蛋白的钝化作用研究初探

被引:17
作者
马晓东
陈珊珊
李重九
机构
[1] 中国农业大学理学院应用化学系
关键词
D O I
暂无
中图分类号
Q946 [植物生物化学];
学科分类号
071001 [植物学];
摘要
This paper studied the deactivation of propionyl shikonin(PS) to tobacco mosaic virus(TMV).After preparation and isolation of TMV coat protein(CP),concentration and purity test were made by SDS-PAGE and MALDI-TOF MS.Bonding of PS and TMV-CP was determined also by MALDI-TOF MS.Peptide mass spectra of trypsin enzymolysis treated by propionyl shikonin were studied.Based on the rate of changes of peptide relative abundance of TMV-CP bonding with PS,the bonding site was computed with SGI workstation,the pKa value was 4.95.
引用
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页码:76 / 79
页数:4
相关论文
共 6 条
[1]
精编分子生物学实验指南.[M].(美)F.M.奥斯伯等主编;马学军;舒跃龙等译校;.科学出版社.2005,
[2]
蛋白质电泳实验技术.[M].郭尧君编著;.科学出版社.2005,
[3]
蛋白质技术手册.[M].汪家政;范明主编;.科学出版社.2000,
[4]
分子病毒学.[M].侯云德著;.学苑出版社.1990,
[5]
植物病毒学.[M].袁维蕃 主编.科学出版社.1985,
[6]
丙酰紫草素对病毒的钝化作用 [J].
李重九 ;
费菁 ;
福士幸治 ;
川端润 ;
田原哲士 ;
水谷纯也 ;
上田一郎 .
中国农业大学学报, 1997, (05) :47-50