以甘露糖作为筛选剂的棉花遗传转化

被引：19

作者：

岳建雄

孟钊红

张炼辉

韩少杰

林亲铁

王巍

机构：

[1] 广东省工程技术研究所

[2] 山西省农科院棉花研究所

[3] 新加坡分子农业生物学院

[4] 广东省工程技术研究所广州

[5] 运城

[6] 新加坡

[7] 广州

来源：

棉花学报 | 2005年 / 01期

关键词：

棉花; 转基因; 甘露糖;

D O I：

暂无

中图分类号：

S562.035.3 [];

学科分类号：

0901 ;

摘要：

以pmi基因作为筛选标记基因,以甘露糖作为筛选剂,通过农杆菌介导法将GFP基因导入棉花细胞并得到再生植株,经过PCR检测、Southern杂交证实外源基因已经整合到棉花基因组中,Westbloting与荧光显微镜检测证明GFP基因得到表达。本文研究讨论了甘露糖作为棉花转化细胞的筛选剂在农杆菌介导的转化中的应用浓度及方法,即:甘露糖的筛选浓度在30～50g·L 1之间,在愈伤组织诱导初期适当低一点,随着愈伤组织的生长而加大筛选浓度。由于甘露糖不利于再生胚的分化,当愈伤组织转入分化培养基时,要以葡萄糖代替甘露糖。

引用

页码：3 / 7

页数：5

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