槲皮素诱导TRAMP-C2细胞凋亡的实验研究

被引：6

作者：

宋明山 ^{[1
]}

邢念增 ^{[2
]}

机构：

[1] 泰安市中心医院泌尿外科

[2] 首都医科大学附属北京朝阳医院泌尿外科

来源：

临床泌尿外科杂志 | 2008年 / 09期

关键词：

前列腺癌; 槲皮素; 基因; 凋亡;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理]; R737.25 [前列腺肿瘤];

学科分类号：

100214 [肿瘤学]; 100806 [中药药理学];

摘要：

目的:探讨槲皮素对转基因前列腺腺癌小鼠C2细胞系(TRAMP-C2)凋亡影响及其作用机制。方法:采用不同浓度的槲皮素处理体外培养的TRAMP-C2细胞,应用MTT法检测槲皮素对TRAMP-C2细胞增殖的影响;采用流式细胞仪检测TRAMP-C2细胞的凋亡率,并应用基因芯片技术筛选槲皮素作用前后TRAMP-C2细胞差异性表达基因。结果:槲皮素能抑制TRAMP-C2细胞生长,诱导TRAMP-C2细胞凋亡,呈剂量及时间依赖性;基因芯片分析发现,应用5×10-5mol/L浓度槲皮素处理48h后的TRAMP-C2细胞有69个基因表达下调,94个基因表达上调,其中血小板反应素表达明显上调,为对照组的36倍。结论:槲皮素能诱导TRAMP-C2细胞凋亡,具有直接抗肿瘤作用,其作用机制可能与上调TRAMP-C2细胞血小板反应素-1的表达有关。

引用

页码：708 / 711

页数：4

共 7 条

[1]

Quercetin downregulates matrix metalloproteinases 2 and 9 proteins expression in prostate cancer cells (PC-3).[J].M. R. Vijayababu;A. Arunkumar;P. Kanagaraj;P. Venkataraman;G. Krishnamoorthy;J. Arunakaran.Molecular and Cellular Biochemistry.2006, 1

[2]

Expression of thrombospondin-1 in prostate-derived cell lines [J].

Bastian, M ;

Steiner, M ;

Schuff-Werner, P .

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2005, 15 (01) :49-56

[3]

Quercetin-induced growth inhibition and cell death in prostatic carcinoma cells (PC-3) are associated with increase in p21 and hypophosphorylated retinoblastoma proteins expression [J].

Vijayababu, MR ;

Kanagaraj, P ;

Arunkumar, A ;

Ilangovan, R ;

Aruldhas, MM ;

Arunakaran, J .

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2005, 131 (11) :765-771

[4]

Inhibition of ErbB-2 and ErbB-3 expression by quercetin prevents transforming growth factor alpha (TGF-α)- and epidermal growth factor (EGF)-induced human PC-3 prostate cancer cell proliferation [J].

Huynh, H ;

Nguyen, TTT ;

Chan, E ;

Tran, E .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2003, 23 (03) :821-829

[5]

Effects of the flavonoid drug Quercetin on the response of human prostate tumours to hyperthermia in vitro and in vivo [J].

Asea, A ;

Ara, G ;

Teicher, BA ;

Stevenson, MA ;

Calderwood, SK .

INTERNATIONAL JOURNAL OF HYPERTHERMIA, 2001, 17 (04) :347-356

[6]

Quercetin inhibits the expression and function of the androgen receptor in LNCaP prostate cancer cells.[J].Nianzeng Xing;Yi Chen;Susan H. Mitchell.Carcinogenesis.2001,

[7]

Flavonoids suppress androgen-independent human prostate tumor proliferation [J].

Knowles, LM ;

Zigrossi, DA ;

Tauber, RA ;

Hightower, C ;

Milner, JA .

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2000, 38 (01) :116-122

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